六味地黄丸(大蜜丸)_中蜂蜜辅料的质量考察(2)糖转化产物5-羟甲基糠醛的测定
Quality investigation of honey in Liuwei Dihuang pill(big honeyed pills)(2) Determination of 5-hydroxymethyl furfural(5-HMF)
分类号：
出版年·卷·期（页码）：2010,30 (12):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立高效液相色谱法测定六味地黄大蜜丸中糖转化产物5-羟甲基糠醛的含量。方法: 采用Agilent TC C18色谱柱,甲醇-0.1%醋酸(3∶[KG-*2]97)为流动相,柱温:30 ℃,流速:1.0 mL·min-1;检测波长:284 nm。结果: 5-羟甲基糠醛进样量在0.00054~2.2 μg范围内线性关系良好(r=1.000),最低检出限为2.1581×10-4μg,加样回收率(n=6)为100.1%(RSD%=1.7%)。结论: 本法简便、准确,可用于六味地黄大蜜丸中5-羟甲基糠醛的含量测定。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish an HPLC method for determination of 5-hydroxymethyl furfural(5-HMF). Method: RP-HPLC was adopted.The separation was performed on Agilent TC C18(250 mm×4.6 mm,5 μm) colum with the mobile phase of methanol-0.1%aqueous acetic acid (3∶[KG-*2]97)at 25 ℃.The detection wavelength was set at 284 nm. Results: The linear ranges were 0.00054-2.2 μg,the lowest detection limit was 2.1581×10-4 μg,The average recovery(n=6) was 100.1%(RSD%=1.7%). Conclusion: A rapid,sensitive and accurate method can provide a scientific basis for the quality control of Liuwei Dihuang (big honeyed pills).
-----参考文献：---------------------------------------------------------------------------------------
欢迎阅读《药物分析杂志》！您是该文第 1125位读者！
