反相高效液相色谱法同时测定黄术健胃片中野黄芩苷、盐酸小檗碱及丹皮酚的含量

目的: 建立同时测定黄术健胃片中野黄芩苷、盐酸小檗碱及丹皮酚的含量测定方法。 方法: 采用RP-HPLC法,Agilent Zorbax SB-C₁₈色谱柱(250 mm×4.6 mm,5 μm);以乙腈-20 mmol·L^-1乙酸铵溶液(用冰醋酸调pH至4.0)为流动相梯度洗脱;流速为1.0mL·min^-1;检测波长为335 nm。 结果: 野黄芩苷的线性范围为0.05~0.26 μg,r = 1.0000,平均回收率为102.2%;盐酸小檗碱的线性范围为0.32~1.62 μg,r = 0.9998,平均回收率为104.1%;丹皮酚的线性范围为0.32~1.62 μg,r=0.9998,平均回收率为99.6%。 结论: 该方法简便、快速、准确,重现性好,可作为该制剂中野黄芩苷、盐酸小檗碱及丹皮酚的含量测定方法。

Objective: To establish a method for the determination of scutellarin,berberine hydrochloride and Paeonol simultaneously. Methods: The RP-HPLC system consisting of a Agilent Zorbax SB-C₁₈ column (250 mm×4.6 mm,5 μm), acetonitrile-20 mmol·L^-1 acetic ammonium(adjusted pH to 4.0 with acetic acid glacial) as mobile phase(using gradient) with flowing rate 1.0mL·min^-1 and detected at 335 nm. Results: The calibration curve of scutellarin was linear from 0.05-0.26 μg with r=1.0000, the average recovery was 102.2%; The calibration curve of berberine hydrochloride was linear from 0.32-1.62 μg with r=0.9998, the average recovery was 104.1%; The calibration curve of paeonol was linear from 0.32-1.62 μg with r=0.9998, the average recovery was 99.6%. Conclusion: The method is simple, quick, accurate and reproducible, and can be used for the quality control of the preparation all-around.