头孢克洛结构测定与分析

目的: 建立药物头孢克洛的结构确证方法。 方法: 通过高效液相色谱-质谱联用仪(HPLC/MS)对头孢克洛进行了纯度测定及质谱分析;通过热分析(DSC、TG)、红外光谱(FT-IR)、核磁共振(NMR)、元素分析(EA)以及X射线粉末衍射(XRD)等分析手段对头孢克洛试样进行结构测定。 结果: 证实头孢克洛的结构为(6R,7R)-7- -3-氯-8-氧代-5-硫杂-1-氮杂双环 辛-2-烯-2-甲酸一水合物。 结论: 本方法测定结果准确,为头孢克洛产品的质量评价提供了较为全面的参考数据。

Objective: To establish a method for the determination of the structure of cefaclor. Method: The structure of cefaclor was analyzed by high performance liquid chromatography-ion trap mass spectrometry(HPLC/MS),differential scanning calorimetry(DSC),thermo gravimetric analyzer(TG),infrared Fourier transform spectroscopy(FT-IR),nuclearmagnetic resonance(NMR),elemental analysis(EA),and the X-ray powder diffraction(XRD). Result: The chemical structure of cefaclor is(6R,7R)-7-{ amino}-3-chloro-8-oxo-5-thia-1-azabicyclo oct-2-ene-2-carboxylic acid monohydrate. Conclusion: The result of the method is accurate and it can provide a comprehensive reference for the quality evaluation of cefaclor.