RP-HPLC法测定骨筋丸胶囊中士的宁的含量

目的: 采用反相高效液相色谱法对骨筋丸胶囊中马钱子所含士的宁进行定量检测,以证明骨筋丸胶囊中士的宁含量测定分析方法能适合制剂检测的要求,确保检验结果的有效性。 方法: 采用十八烷基硅烷键合硅胶为填充剂(Kromasil 250 mm×4.6 mm,5 μm)的色谱柱;流动相为乙腈0.01 mol·L^-1庚烷磺酸钠与0.02 mol·L^-1磷酸二氢钾等量混合(用10%磷酸调节pH至2.8)(21∶ 79);检测波长:254 nm;流速:1.0 mL·min^-1。 结果: RP-HPLC士的宁在0.2128~1.064 μg范围内线性关系良好(r=0.9995),平均回收率为98.74%。 结论: 该方法准确可靠地对骨筋丸胶囊进行定性、定量检测,能有效地控制制剂的质量,使其更有利于指导生产,保证产品质量。

objective: For prove Gujinwan capsules sergeant would rather content determine analytical method can suitable for requirement that preparation measure,guarantee the validity of the test result. Methods: Adopt high-efficient liquid phase chromatogram law Semen Strychni would rather carry on quantitative to measure while being including person to Gujinwan capsules inside out.Chromatogram column:C₁₈ alkyl silane key shut colloidal silica for pack pharmaceutical (Kromasil 250 mm×4.6 mm,5 μm ); The looks of flowing:Second nitrile-0.01 mol·L^-1age alkane sulphur sour sodium and 0.02 mol·L^-1phosphoric acid two hydrogen potassium mix equivalently (regulate with 10 phosphoric acid pH go to 2.8)( 21∶ 79);Measure the wavelength:254 nm;Velocity of flow:1.0 mL·min^-1. Result: RP-HPLC person would rather be in house phone relation of 0.2128-1.064 μg range was good (r =0.9995),the average rate of recovery was 98.74%. Conclusion: This method is very accurate to determine the Gujinwan capsules,measure quantitativly reliably,can control the quality of preparation effectively,make it furthermore help guidance production,guarantee product quality.