肝素钠和多硫酸软骨素免疫原性的实验研究
Study on immunogenicity of heparin sodium and chondroitin polysulfate
分类号：
出版年·卷·期（页码）：2010,30 (10):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 观察肝素钠和多硫酸软骨素是否具有免疫原性。 方法: 分别用肝素钠、含硫酸软骨素杂质肝素钠和多硫酸软骨素与福氏佐剂混匀免疫家兔,采用酶联免疫吸附试验(ELISA)和琼脂免疫扩散试验检测家兔血清中的抗体。 结果: ELISA 法未检测到血清中的抗体;琼脂免疫扩散试验血清72 h扩散观察未出现沉淀线,没有形成抗原抗体复合物。 结论: 肝素钠和多硫酸软骨素对家兔无免疫原性。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To study the immunogenicity of heparin sodium and chondroitin polysulfate. Methods: Rabbits were immunized with a mixture of heparin sodium,heparin sodium with impurity of chondroitin polysulfate,chondroitin polysulfate and Freund adjuvant.The antibodies of the rabbit were detected by enzyme linked immunosorbent assay (ELISA) and agar-immune diffusion test. Results: No antibody was found in all rabbits by ELISA.The agar-immune diffusion test indicated that no antigen-antibody compound formed in 72 h. Conclusion: Heparin sodium and chondroitin polysulfate have no immunogenicity on rabbits.
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