毛细管电泳-化学修饰电极电致化学发光法测定琥乙红霉素

目的: 建立一种毛细管电泳电致化学发光测定琥乙红霉素的新分析方法。 方法: 利用铕离子掺杂普鲁士蓝化学修饰铂电极对联吡啶钌的电催化氧化作用可显著增强电致化学发光法检测琥乙红霉素的灵敏度,据此建立了毛细管电泳-化学修饰电极电致化学发光检测琥乙红霉素的分析新方法。 结果: 在优化的实验条件下,发光强度与琥乙红霉素的浓度在1~100 μg·mL^-1 (r=0.9993)之间呈良好线性关系,检出限为0.25 μg·mL^-1 (S/N=3)。对含10.0 μg·mL^-1琥乙红霉素标样连续测定5次,发光强度和迁移时间的RSD分别为2.2%,0.81% 。 结论: 本法具有分离度好,灵敏高,分析试样无需复杂处理可直接进行测定等优点,可对药物制剂和合成人工尿样中琥乙红霉素含量进行简便、快速的测定。

Objective: To establish a simple and novel method for the determination of EES in pharmaceutical preparations. Methods: An Eu-PB modified platinum electrode as work electrode of CE-ECL detector,showed excellent electrocatalytic activity for the oxidation of Ru(bpy)²⁺₃.Therefore,the ECL intensity of Ru(bpy)²⁺₃ could be markedly enhanced for measuring EES.Hence,a new method for the determination of EES by capillary electrophoresis coupled with chemically modified electrode electrochemiluminescence detection was developed. Results: Parameters affecting separation and detection were also optimized.The linearity was in the range of 1 μg·mL^-1 to 100 μg·mL^-1 and detection limit was 0.25 μg·mL^-1 (S/N=3).When the concentration of EES was 10.0 μg·mL^-1,the RSD value of migrate time and peak high,were 2.2% and 0.81% for six repeating detection,respectively. Conclusion: This method has fast,sensitive,accurate advantage and sample can be injected without complicated pretreatment.It has been applied to the determination of EES in erythromycin ethyisuccinate granules drug and human urine sample.