HPLC法测定一枝黄花中芦丁的含量
HPLC determination of rutin in Solidago decurrens Lour.
分类号：
出版年·卷·期（页码）：2010,30 (9):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立高效液相色谱法测定一枝黄花中芦丁含量。 方法: 采用Hypersil ODS2 C18(4.6 mm×250 mm,5 μm)色谱柱,流动相为乙腈-甲醇-冰醋酸(16∶ 8∶ 76),流速1 mL·min-1,检测波长360 nm。 结果: 芦丁进样量在0.199~3.58 μg范围内线性关系良好(r=0.9993);平均回收率(n=6)为99.2%,RSD为2.1%。 结论: 本分析方法简便、准确,可作为该药材的定量分析方法。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To develop an HPLC method for determination of rutin in Solidago decurrens Lour.from different source. Methods: Hypersil C18(4.6 mm×250 mm,5 μm) column was adopted,the mobile phase consisted of acetonitrile-methanol-acetic acid glacial(16∶ 8∶ 76) at the flow rate of 1.0 mL·min-1,and the detection wavelength was 360 nm. Results: The linear range of rutin was 0.199-3.58 μg(r=0.9993),and the recovery of this method was 99.2% with RSD of 2.1%. Conclusion: The method is convenient,accurate,and can be used for determination of the medicinal herb.
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