RRLC法与HPLC法在红参和西洋参人参皂苷含量测定中的分析比较

目的: 应用超高速液相色谱(RRLC)法、常规高效液相色谱(HPLC)法和Merck C₁₈整体柱HPLC法测定红参、西洋参中人参皂苷Rg₁、Re和Rb₁含量的分析比较。 方法: 分别采用Agilent Zorbax Eclipse XDB-C₁₈(50 mm×4.6 mm,1.8 μm)色谱柱、Diamonsil C₁₈(200 mm×4.6 mm,5 μm)色谱柱和Merck C₁₈(100 mm×4.6 mm,2 μm)整体柱,以乙腈-水(红参)和乙腈-0.1%磷酸溶液(西洋参)为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长203 nm,以外标法进行定量。 结果: RRLC法和Merck C₁₈整体柱的应用与常规C₁₈柱的HPLC法相比,对红参、西洋参中人参皂苷Rg₁、Re和Rb₁的含量测定结果差异不大,但可显著缩短分析检验时间。 结论: RRLC法和Merck C₁₈整体柱HPLC法简便快捷,分离度好,结果准确,重复性好。

Objective: To compare a rapid resolution liquid chromatography(RRLC) and two HPLC methods for determining the compounds ginsenoside Rg₁,Re and Rb₁in Radix et Rhizoma Ginseng Rubra and Radix Panacis Quinuefolii. Methods: The separation was performed on Agilent Zorbax Eclipse XDB-C₁₈(50 mm×4.6 mm,1.8 μm)column,Diamonsil C₁₈(200 mm×4.6 mm,5 μm) column and Merck monolithic C₁₈ column(100 mm×4.6 mm,2 μm) by gradient elution.The mobile phase consisted of acetonitrile-water for Radix et Rhizoma Ginseng Rubra or acetonitrile-0.1% phosphoric acid for Radix Panacis Quinuefolii.The flow rate was 1.0 mL·min^-1 with detection wavelength 203 nm. Results: The contents of Rg₁,Re and Rb₁ determined by the developed methods showed no differences,but the analysis time was greatly shortened. Conclusion: The developed RRLC and HPLC with Merck C₁₈ column methods are rapid,accurate and reproducible for quality control of Radix et Rhizoma Ginseng Rubra and Radix Panacis Quinuefolii.