LC-MS/MS法快速测定中成药、保健食品中非法添加的36种化学成分
LC-MS/MS screening for 36 chemical components in health food and Chinese traditional patent medicine
分类号:
出版年·卷·期(页码):2010,30 (8):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 建立中成药、保健食品中非法添加的36种解热镇痛、抗炎化学成分的快速测定方法。 方法: 采用液相色谱-串联质谱法(LC-MS/MS),以SunfireTM C18(2.1 mm×100 mm,5.0 μm)为分析柱,流动相采用甲醇-0.1%甲酸(75∶25),电喷雾电离(ESI) 、多反应监测(MRM) 扫描方式,对中成药、保健食品中非法添加的36种化学成分进行快速定性定量分析。 结果: 建立了快速分离检测中成药、保健食品中36种非法添加成分的测定方法,检测限低于20 ng。 结论: 该方法专属性强、灵敏度高,适用于解热镇痛、抗炎类中成药、保健品中非法添加成分的定性定量分析。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening 36 chemical components in health food and Chinese Traditional Patent Medicine. Methods: This method involves liquid chromatography-tandem mass spectrometry,the separation was conducted by SunfireTM C18(2.1 mm ×100 mm,5.0 μm).The mobile phase consisted of 0.1% formic and methanol (25∶75).The target compounds were analyzed with LC-MS/MS-ESI.Multiple-reaction monitoring (MRM) was used to screen 36 chemical components in health food and Chinese Traditional Patent Medicine. Results: A fast and sensitive liquid chromatography mass spectrometric (LC/MS) method for the simultaneous screening of 36 chemical components was described.The LOD of these substances were below 20 ng. Conclusion: The method was sufficiently selective and sensitive to detect chemical components in health food and Chinese Traditional Patent Medicine.
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