HPLC和UPLC法对比测定千里香药材中九里香酮的含量

目的: 用高效液相色谱(HPLC)法与超高效液相色谱(UPLC)法对比测定千里香药材中九里香酮的含量。 方法: 分别建立了HPLC和UPLC测定千里香药材中九里香酮含量的方法。HPLC法为AKZONOBEL Kromasil 100-5 C₁₈ (4.6 mm×250 mm,5 μm)色谱柱,以乙腈-水(30∶ 70)为流动相,流速1 mL·min^-1,检测波长337 nm,柱温30 ℃;UPLC法为Waters Acquity BEH C₁₈(2.1 mm×50 mm,1.7 μm)色谱柱,以乙腈-水(23∶ 77)为流动相,流速0.5 mL·min^-1,检测波长337 nm,柱温30 ℃。 结果: 2种方法所得含量测定结果一致。HPLC法和UPLC法加样回收率的RSD分别是1.8%和1.7%。 结论: HPLC和UPLC法测定千里香中九里香酮的含量,方法简便,结果准确可靠。UPLC较HPLC法更加简便迅速,既达到了分析的高通量,得到更高的分析灵敏度,又大大提高了分析速度,减少了有机溶剂的消耗。UPLC法能够成功替代HPLC法,为千里香药材质量控制提供新的检测方法。

Objective: To compare the methods—HPLC and ultra performance liquid chromatography(UPLC) for the determination of 6,8,3',4',--tetramethoxy flavone in Murraya paniculata (L.) Kack. Method: Respectively establish the HPLC and UPLC methods for the determination of 6,8,3',4',--Tetramethoxy flavone in Murraya paniculata (L.) jack.A AKZONOBEL Kromasil 100-5 C₁₈column (4.6 mm×250 mm,5 μm)was adopted with methanol-water (30∶ 70)as mobile phase at a flow rate of 1 mL·min^-1;detective wavelength was set at 337 nm and column temperature was 30 ℃ in HPLC.A Waters Acquity BEH C₁₈ column(2.1 mm×50 mm,1.7 μm)was adopted,with acetonitrile-water (23∶ 77)as mobile phase at a flow rate of 0.5 mL·min^-1;detective wavelength was set at 337 nm and column temperature was 30 ℃ in UPLC. Results: Both of the two methods for the determination had the same results.The RSD of the average recovery of HPLC and UPLC were 1.8% respectively,1.7%. Conclusion: HPLC and UPLC methods for the determination of 6,8,3',4',--tetramethoxy flavone in Murraya paniculata (L.) Jack are simple,the results are accurate and reliable.The major advantages of UPLC,over HPLC are the speed of analysis,the narrower peaks(giving increased signal-to-noise ratio),UPLC can not only increase separation speed and efficiency,but also reduce solvent consumption and analysis time.UPLC as a new detection method can replace HPLC for the quality control of Murraya paniculata (L.) Jack.