高效液相色谱法测定小儿润肺止咳口服液中橙皮苷和黄芩苷的含量

目的: 建立高效液相色谱法测定小儿润肺止咳口服液中橙皮苷和黄芩苷含量的方法。 方法: 色谱柱采用十八烷基硅烷键合硅胶为填充剂(150 mm×4.6 mm,5 μm)。流动相:A为0.5% 磷酸溶液(含5%甲醇),B为甲醇,梯度洗脱。洗脱程序:0~45 min,A由90%~60%,B由10% ~ 40%,流速:1 mL·min^-1,检测波长284 nm。 结果: 以峰面积(X)对进样浓度(Y,μg·mL^-1)线性回归,橙皮苷回归方程:Y =0.05488X +0.01433,线性范围2.690 ~ 33.63 μg·mL^-1,黄芩苷回归方程:Y=0.03710X+0.03698,线性范围7.969 ~ 99.61 μg·mL^-1,r=0.9999。橙皮苷回收率为97.2%;RSD为1.1%;黄芩苷回收率为101.3%;RSD为0.60%。 结论: 本方法操作简便,测定结果准确,重复性好,可用于小儿润肺止咳口服液橙皮苷和黄芩苷的含量测定。

Objective: To develop an HPLC quantitative method for the determination of hesperidin and baicalin in Xiaoer Runfeizhike oral liquid. Methods: Column C₁₈(150 mm×4.6 mm,5 μm),0.5% phosphoric acid(include 5% methanol)as mobile phase A,methanol as mobile phase B at flow rate of 1 mL·min^-1.Gradient elution:0-45 min,A:90%→60%,B:10%→40%;and monitored at 284 nm. Results: The regress equation for hesperidin was Y=0.05488X+0.01433,r=0.9999 and the linear range was 2.690-33.63 μg·mL^-1,The average recovery of hesperidin was 97.2%,RSD 1.1%.The regress equation for baicalin was Y=0.03710X+0.03698,r=0.9999 and the linear range was 7.969-99.61 μg·mL^-1,The average recovery of baicalin was 101.3%,RSD 0.60%. Conclusion: This method is simple,time-saving and accurate.It can be used for routine analysis of hesperidin and baicalin in Xiaoer Runfeizhike Koufuye.