RP-HPLC法测定角花胡颓子中齐墩果酸与熊果酸的含量

目的: 建立同时测定角花胡颓子中齐墩果酸与熊果酸含量的RP-HPLC法。 方法: 采用Diamonsil C₁₈(250 mm×4.6 mm,5 μm),以甲醇－0.5%乙酸铵(85∶ 15)为流动相,检测波长为206 nm,流速为1.0 mL·min^-1,柱温30 ℃。 结果: 齐墩果酸进样量在0.06~0.36 μg(r = 0.9995)、熊果酸进样量在0.65~3.9 μg (r= 0. 9997)范围内,线性关系良好,齐墩果酸与熊果酸的平均加样回收率分别为99.2％和102.5％,RSD分别为8.4%和6.0%(n=9),重复性试验的含量的RSD分别为6.9%和4.2%(n=6)。 结论: 该法简便、快速、准确,可用于角花胡颓子的质量控制。

Objective: To establish an RP-HPLC for determination of oleanolic acid and ursolic acid in Elaeagnus gonyanthes Benth. Methods: The analytical column was Diamonsil C₁₈ column(250 mm×4.5 mm, 5 μm). The mobile phase consisted of methanol- 0.5% ammonium acetate (85∶ 15). The detective wavelength was 206 nm. The flow rate was 1.0 mL·min^-1 and the column temperature was 30 degrees. Results: Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.36 μg ( r = 0. 9995) and 0.65-3.9 μg(r=0. 9997). The average recovery of oleanolic acid was 99.2％ with relative standard deviation (RSD ) of 8.4% and the ursolic acid was102.5％with RSD of 6.0%(n=9), the reproducibility test of content was 6.9% and 4.2%(n=6). Conclusion: This method is simple, quick, accurate and can be used for quality control of Elaeagnus gonyanthes Benth.