RP-HPLC同时测定管花肉苁蓉中4种苯乙醇苷的含量

目的: 建立RP-HPLC同时测定管花肉苁蓉药材中松果菊苷、管花苷A、毛蕊花糖苷和异毛蕊花糖苷含量的方法。 方法: 采用Megres-C₁₈(250 mm×4.6 mm,5 μm)色谱柱,柱温30 ℃;流动相为甲醇(A)-0.1%甲酸(B),梯度洗脱(0 min,30% A;30 min,40% A;40 min,45% A),流速1.0 mL·min^-1;检测波长330 nm。 结果: 在上述色谱条件下,松果菊苷、管花苷A、毛蕊花糖苷和异毛蕊花糖苷获得良好分离;进样量分别在0.732~11.7 μg(r=0.9991)、0.216~3.45 μg(r=0.9995)、0.209~3.35 μg(r=0.9991)、0.151~2.42 μg(r=0.9993)范围内呈良好的线性关系;平均加样回收率(n=9)分别为101.8%,98.8%,101.1%,99.9%;精密度试验的RSD小于0.65%;重复性试验的RSD小于2.4%。 结论: 该方法简便、准确,重复性好,为管花肉苁蓉药材的质量控制提供可借鉴的分析方法。

Objective: To simultaneous determine the contents of echinacoside,tubuloside A,acteoside and isoacteoside in rhizome of Cistanche tubulosa (Schrenk) Wight by RP-HPLC. Methods: An external standard method by RP-HPLC with Megres-C₁₈ (250 mm×4.6 mm,5 μm) as fixed phase at the temperature 30 ℃.The mobile phase consisted of methanol(A)-0.1% aqueous methanoic acid(B) with the gradient elution (0 min,30%A;30 min,40%A;40 min,45%A),the flow rate:1.0 mL·min^-1.The absorbance was monitored 330 nm. Results: Echinacoside,tubuloside A,acteoside and isoacteoside were well separated by this method.Linearities of echinacoside,tubuloside A,acteoside and isoacteoside were good in the ranges of 0.732-11.7 μg(r=0.9991),0.216-3.45 μg(r=0.9995),0.209-3.35 μg(r=0.9991),0.151-2.42 μg(r=0.9993),respectively.The average recoveries(n=9) of four phenylethanoid glycosides were 101.8%,98.8%,101.1%,99.9%,respectively.All of RSDs of precision(n=6) and repeatability(n=6) were less than 0.65% and 2.4%. Conclusion: The method is simple,accurate,reproducible,and can be used for quality control of Cistanche tubulosa (Schrenk) Wight.