肠炎宁糖浆定性定量方法的研究___

目的: 建立肠炎宁糖浆定性定量方法。方法:采用薄层色谱法对处方中地锦草、黄毛耳草进行定性鉴别;采用高效液相色谱法测定处方中地锦草、黄毛耳草的有效成分没食子酸的含量,色谱柱为Diamonsil C₁₈色谱柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.5%磷酸溶液(10∶90),流速1.0 mL·min^-1,检测波长270 nm,柱温30 ℃。结果:在薄层色谱中可检出地锦草、黄毛耳草;高效液相色谱法测定,没食子酸进样量在0.05158~1.037 μg范围内与峰面积呈良好的线性关系(r=0.9998),平均回收率(n=6)为101.9%(RSD=2.1%)。结论:所建立的方法可准确地进行定性、定量检测,可用于该制剂的质量控制。

Objective: To establish the methods of qualitation and quantitation of Changyanning syrup.
Methods:Adopting TLC to make a qualitative identification of Herba Euphorbiae Humifusae and Herba Hedyotidis Chrysotrichae in the prescription.Using HPLC to determine gallic acid’s content of Herba Euphorbiae Humifusae and Herba Hedyotidis Chrysotrichae in the prescription.Diamonsil C₁₈ column(250 mm×4.6 mm,5 μm) was adopted and the mobile phase was methanol-0.5% phosphoric acid(10∶90) at the flow rate of 1.0 mL·min^-1,the detection wavelength was 270 nm and column temperature was 30 ℃. Results:Herba Euphorbiae Humifusae and Herba Hedyotidis Chrysotrichae could be detected by TLC. The gallic acid showed good linear relationship(r=0.9998) in the range of 0.05158-1.037 μg  and the average recovery(n=6)was 101.9% with RSD of 2.1%.Conclusion:What the methods were set up can make the qualitative and quantitative detection accurately, and use the quality control of the pharmaceutics.