南葶苈子药材含量标准的研究___
Research on chemical content standard of Semen Descurainiae
分类号：
出版年·卷·期（页码）：2010,30 (5):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立高效液相色谱法测定南葶苈子药材中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的含量,确定南葶苈子药材的含量标准。 方法 :供试品溶液制备方法为70%甲醇回流1 h,色谱柱为Venusil MP C18(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1%醋酸水溶液(11∶ 89),流速1.0 mL·min-1,检测波长254 nm,柱温25 ℃。 结果 :槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷进样量在0.156~1.56 μg范围内与峰面积呈良好的线性关系;加样回收率为99.12%,RSD=1.3%(n=6)。 结论 :本方法分离度较好,具有很好的重现性和稳定性。综合28批南葶苈子药材含量测定结果,槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的含量限度应不低于0.075%。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish a method to determine the content of quercetin-3-O-β-D-glucopyranosyl- 7-O-β-D-gentiobioside in Semen Descurainiae,and to make the chemical content standard of the herb. Methods: The method of sample solution preparing was refluxing the herb in 70% methanol for 1 h.In HPLC method,the self-made quercetin-3-O-β-D-glucopyranosyl-7-O-β-D-gentiobioside was used as reference substances.The chromatographic conditions were as follows:MP C18 column(250 mm×4.6 mm,5 μm),acetonitrile-0.1% acetic acid solution(11∶ 89)as mobile phase,the follow rate 1.0 mL·min-1,detection wavelength at 254 nm,the temperature of column 25 ℃. Results: The linear range was 0.156-1.56 μg for the reference compound.The average recovery was 99.12%with RSD of 1.3%(n=6). Conclusion: The method is simple and accurate,with good separation,repeatability and stability.Through the results of 28 batches of the herb,the content of quercetin-3-O-β-D-glucopyranosyl-7-O-β-D-gentiobioside in Semen Descurainiae should not be less than 0.075%.
-----参考文献：---------------------------------------------------------------------------------------
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