HPLC法测定不同来源杠板归中咖啡酸的含量___
HPLC determination of caffeic acid in Polygonum perfoliatum L. from different source
分类号：
出版年·卷·期（页码）：2010,30 (5):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立HPLC法测定不同来源的杠板归药材中咖啡酸含量。 方法: 采用Hypersil ODS 2 C18(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-0.4%磷酸(9∶ 91)为流动相,流速1.0 mL·min-1,检测波长323 nm。 结果: 咖啡酸进样量在0.0225~0.607 μg范围内线性关系良好(r=0.9999);平均回收率(n=6)为 100.5%,RSD为 0.74%。 结论: 本法简便,准确,可作为该药材的定量分析方法。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To develop an HPLC method to determine the contents of caffeic acid in Polygonum perfoliatum L.from different source. Methods: Hypersil ODS 2 C18(4.6 mm×250 mm,5 μm) was adopted; The mobile phase consisted of acetonitrile-0.4% phosphoric acid(9∶ 91 ) at the flow rate of 1.0 mL·min-1; The detection wavelength was 323 nm. Results: The linear range of caffeic acid was 0.0225-0.607 μg,the recovery(n=6) of this method was 100.5% and RSD was 0.74%. Conclusion: The method is convenient,accurate and can be applied in quantitative determination of the medicinal herb.
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