SPE-HPLC法测定藿香正气水中厚朴酚与和厚朴酚的含量___

目的: 对藿香正气水样品前处理的中国药典方法进行改进,建立采用SPE-HPLC法测定藿香正气水中厚朴酚与和厚朴酚含量的分析方法。 方法: 采用自制硅胶SPE小柱提取纯化样品。采用C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以甲醇-水-乙腈-冰醋酸(70:25:5:0.25)为流动相,流速1.0 mL·min^-1,检测波长294 nm,柱温为室温。 结果: SPE-HPLC法测得厚朴酚浓度在4.0~80 μg·mL^-1(r=0.9998),和厚朴酚浓度在2.0~40 μg·mL^-1(r=0.9998)范围内与其峰面积线性关系良好;厚朴酚的平均回收率(n=5)为99.9%(RSD=0.7%),和厚朴酚的平均回收率(n=5)为99.5%(RSD=0.5%)。 结论: 该方法环保、简便、准确,重现性好,能有效地控制藿香正气水的质量。

Objective: Based on the improvement of the sample pretreatment method in the criterion,an SPE-HPLC method for determining the contents of magnolol and honokiol in Huoxiang Zhengqi solution was established. Methods: Samples were purified by silica gel SPE column.The HPLC column used in the experiment was C₁₈(250 mm×4.6 mm,5 μm),and the mobile phase was methanol-water-acetonitrile-glacial acetic acid(70:25:5:0.25)at the flow rate of 1.0 mL·min^-1;The detection wavelength was 294 nm,and the experiment was accomplished at room temperature. Results: The contents of magnolol and honokiol determined by SPE-HPLC method had good linear relationship with the peak areas when their concentrations at 4.0-80.0 μg·mL^-1(r=0.9998),2.0-40.0 μg·mL^-1(r=0.9998),respectively;The average recovery(n=5)of magnolol was 99.9%(RSD=0.7%),and that of honokiol was 99.5%(RSD=0.5%). Conclusion: The method is environmental protection,simple,accurate,with good reproducibility,and it can be used for the quality control of Huoxiang Zhengqi solution.

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