白屈菜药材的UPLC特征图谱建立及生物碱类成分的QTOF-MS分析___

目的: 建立白屈菜药材的UPLC-UV特征图谱并用QTOF-MS技术分析其生物碱类成分。 方法: 采用ACQUITY UPLC BEH C₁₈(2.1 mm×100 mm,1.7 μm)色谱柱,以乙腈-10 mmol·L^-1醋酸铵缓冲液(用醋酸调节至pH 3.0)为流动相梯度洗脱,流速0.4 mL·min^-1,检测波长280 nm,柱温35 ℃,对10批白屈菜药材的特征图谱进行比较研究,用UPLC-ESI-QTOF-MS技术对其共有峰进行分析。 结果: 获得白屈菜药材的UPLC特征图谱,共有峰17个,鉴定出14个生物碱成分,其中6个通过对照品对照确认,8个通过质谱信息推测。 结论: 建立的UPLC-UV特征图谱可用于白屈菜质量评价。

Objective: To establish the UPLC specific chromatogram of Chelidonium majus L.and analyze the alkaloids compositions by QTOF-MS. Methods: The samples were separated by ACQUITY UPLC BEH C₁₈ column(2.1 mm×100 mm,1.7 μm)and eluted with acetonitrile-10 mmol ·L^-1 ammonium acetate buffer(adjusted to pH 3.0 with acetic acid)at a flow rate of 0.4 mL·min^-1.The detection wavelength was 280 nm and column temperature was 35 ℃.In the condition,10 samples of Chelidonium majus were analyzed.Common peaks were analyzed by UPLC-ESI-QTOF-MS. Results: The specific chromatograms of Chelidonium majus L.were obtained.There were 17 common peaks.Fourteen alkaloids compositions were identified.Among them,6 compositions were identified by direct comparison with the reference substances and others were identified by MS and MS² data. Conclusion :UPLC specific chromatogram facilitates the evaluation of the quality of Chelidonium majus L..

[1]. ChP(中国药典).1977.Vol Ⅰ(一部):176
[2]. Editorial Board of Chinese Materia Medica,State Administration of Traditional Chinese Medicine of the Peoples Republic of China(中华人民共和国国家中医药管理局《中华本草》编委会).Chinese Materia Medica(中华本草).Vol 9(第9卷).Shanghai(上海):Shanghai Science and Technology Publishers(上海科学技术出版社),1999.616
[3]. NIU Chang-qun(牛长群),HE Li-yi(何丽一).Alkaloid content change rule research of Chelidonium majus(中药白屈菜生物碱含量变化规律的研究).Chin Tradit Herb Drugs(中草药),1994,25(8):409
[4]. TAO Ling(陶玲),QU Cai-hong(曲彩红),HUANG Ji-wei(黄际薇),et al. HPLC fingerprint of Chelidonium majus injection(白屈菜注射液HPLC指纹图谱研究).J Chin Med Mater(中药材),2004,27(5):376
[5]. LI Xiao-meng(李晓蒙),ZHU Sheng-hua(朱盛华),LIAO Hua-wei(廖华卫).HPLC fingerprint of Chelidonium majus(白屈菜药材HPLC指纹图谱研究).Nat Prod Res Dev(天然产物研究与开发),2002,14(6):33
[6]. ZHOU Jin-yun(周金云),CHEN Bi-zhu(陈碧珠),TONG Xiao-jie(佟晓杰),et al.Chemical study of the alkaloid constituents from Chelidonium majus(白屈菜生物碱的化学研究).Chin Tradit Herb Drugs(中草药),1989,20(4):1
[7]. Matile P.Localization of alkaloids and mechanism of their accumulation in vacuoles of Cheidonium majus laticifers.Nova Acta Leopold Suppl,1976,7:139
[8]. FENG Rui-zhi(冯瑞芝),LIAN Wen-yan(连文琰),FU Gui-xiang(傅桂香),et al.Chemotaxonomy and resource utilization of the tribe Chelidonieae(Papaveraceae)(罂粟科白屈菜族的化学分类及资源利用).Acta Phytotaxon Sin(植物分类学报),1985,23(1):36
[9]. Slavík J,SlavíkováL.Minor alkaloids from Cheldonum majus L.Collect Czech Chem Commun, 1977,42 :
[2686]. 10 LI Ya-wei(李亚伟),FANG Qi-cheng(方起程).Isoquinoline alkaloid from Corydalis esquirolii Levl.(籽纹紫堇中的异喹啉类生物碱).Chin Tradit Herb Drugs(中草药),1991,22(11):486
[11]. WANG Heng-shan(王恒山),YANG Hai-rong(杨海荣).The alkaloids of Corydalis dasypterm Maxim.(迭裂黄堇生物碱的研究).Nat Prod Res Dev(天然产物研究与开发),1997,9(1):37
[12]. DU Fang-lu(杜方麓),CHEN Sheng-huang(陈胜璜),YANG Chang-ming(阳长明),et al.Eomecon chionantha Hance(血水草化学成分研究). Chin Tradit Herb Drugs(中草药),1993,24(4):177
[13]. KUANG Hai-xue(匡海学),DONG Xiao-ping(董小萍),SHI Ren-bing(石任兵).Traditional Chinese Medicine Chemistry(中药化学).Beijing(北京):China Traditional Chinese Medicine Publishing House(中国中医药出版社),2003.341