HPLC法测定布格呋喃中的对映体___

 

 

目的：建立HPLC法测定布格呋喃中对映体的含量。方法：用Daicel Chiralcel OD手性色谱柱，正己烷为流动相，流速0.5 mL·min-1，柱温30 ℃，进样体积10 μL，检测波长196 nm。结果：在上述色谱条件下，布格呋喃与对映体的分离度为4.24。对映体在1~20 μg·mL^-1范围内与峰面积呈线性关系( r=0.9999)，检测限为3 ng，定量限为10 ng。加样回收率为101.0%，RSD为1.4%（n=9）。布格呋喃中未检出对映体。结论：方法快速简便，实现了布格呋喃与对映体的手性分离，适用于布格呋喃中对映体的检查。

 

Objective:To establish an HPLC method for dermination of enantiomer as impurities in buagafuran.Methods：The Daicel Chiralcel OD column was used with n-hexane as mobile phase，flow rate was 0.5 mL·min^-1，column temperature was 30 ℃，injection volume was 10 μL and wavelength was 196 nm.Results：Under this chromatographic condition，the resolution of buagafuran and its enantiomer was 4.24.Enantiomer showed a good linearity in the range of 1-20 μg·mL^-1 (r=0.9999).The limits of detection and quantitation of the enantiomer were 3 ng and 10 ng，respectively.The average recovery and RSD were 101.0% and 1.4%(n=9).There was no enantiomer being detected in buagafuran.Conclusions：The method is rapid,simple and can be used for separation and determination of enantiomer in buagafuran.

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