Objective:To establish the qualitative and quantitative analysis method for the total flavonols in the flowers of Rosa rugosa.Methods:To detect quercetin and kaempferol by silica TLC with toluene-ethyl formate-formic acid(10∶8∶1) as developing solvent and 3% aluminium trichloride alcoholic solution as visualization reagent;To determine the content of quercetin and kaempferol by HPLC,with C18 column Phenomenex Luna(250 mm×4.6 mm,5 μm) and mobile phase methanol-0.4% phosphoric acid(60∶40),at a flow rate of 1 mL·min-1 and detective wavelength 360 nm for assay.Results:Quercetin and kaempferol were detected by silica gel TLC.By HPLC,the linear range of quercetin was 006-0.6 μg with a correlation coefficient 0.9996,the average recovery(n=5) was 99.2% with RSD of 3.9%;the linear range of kaempferol was 0.02-0.2 μg with a correlation coefficient 0.9993,the average recovery(n=5) was 98.5% with RSD of 4.8%.Conclusion:Qualitative and quantitative analysis method for the total flavonols in the flowers of Rosa rugosa has been built up for the first time,the results also provide a basis for the quality control of Rosa rugosa
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