HPLC法测定金荞麦片中原儿茶酸和表儿茶素的含量___

 

 目的：建立高效液相色谱法测定金荞麦片中原儿茶酸和表儿茶素的含量。方法：采用Agilent TC-C18（250 mm×4.6 mm，5 μm）色谱柱，乙腈-水（pH 3.0）（8∶92）为流动相，检测波长204 nm，流速1.5 mL·min-1，柱温35 ℃，采用外标法计算含量。结果：原儿茶酸在0.07812~1.5624 μg·mL-1范围内线性关系良好(r=0.9999)、表儿茶素在0.1171~2.342 μg·mL-1范围内线性关系良好（r=0.9999）。结论：本法简便、可靠，专属性强，重复性好，可用于金荞麦片的质量控制。

 

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Objective:To establish an HPLC method for the determination of protocatechuic acid and (-)-epicatechin in Jinqiaomai tablets.Methods:The Agilent TC-C18（250 mm×4.6 mm，5 μm） was column adopted with the mobile phase consisted of acetonitrile-water(pH 3.0）（8∶92） at the flow rate of 1.5 mL·min-1.The wavelength was set at 204 nm.The column temperature was 35 ℃.Caculation was processed by external standard method.Results:The linear range was 0.07812-1.5624 μg·mL^-1 (r=0.9999)for protocatechuic acid and 0.1171-2.342 μg·mL^-1 (r=0.9999)for (-)-epicatechin.Conclusion:The method is simple,reliable,accurate and reproducible in quality control of Jinqiaomai tablets.

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