紫外波长切换高效液相色谱法同时测定壮骨关节丸中7种有效成分的含量___

 

 

 

目的：采用紫外波长切换高效液相色谱法，建立同时测定壮骨关节丸中7种有效成分含量的方法。方法：色谱柱Zorbax SB-C₁₈（4.6 mm×250 mm,5 μm）；流动相0.05%甲酸水溶液-乙腈，梯度洗脱；切换时间和检测波长：0~25 min，283 nm；25~30 min，365 nm；30~32 min，270 nm；32~37 min，246 nm；37~65 min，322 nm；柱温：40 ℃；流速：1.0 mL· min^-1。结果：柚皮苷、槲皮素、淫羊藿苷、补骨脂素、异补骨脂素、芒柄花素和蛇床子素在相应的浓度范围内与峰面积呈良好线性关系，相关系数均大于0.9997；平均回收率（n=6）在97.8%~104.3%之间，RSD均小于4.0%。结论：本方法简便快速、准确、精密度高、重复性好，适用于壮骨关节丸的质量控制。

 

 

Objective:To establish a method for simultaneous determination of seven active components in Zhuanggu Guanjie pills using switching UV wavelength.Method:The HPLC analysis performed on a Zorbax SB-C₁₈column with the mobile phase composed of acetonitrile and 0.05% formic acid water in gradient elution.Detection wavelength was set at 283 nm in 0-25 min,365 nm in 25-30 min,270 nm in 30-32 min,246 nm in 32-37 min,322 nm in 37-65 min.The column temperature was maintained at 40 ℃.The flow rate was fixed at 1.0 mL· min^-1.Results:Naringin,quercetin,icariin,psoralen,isopsoralen,formononetin and osthole showed good linearity over the tested ranges (r≥ 09997).The average recoveries were ranged from 97.8 to 104.3% with RSDs ≤ 4.0%.Conclusion:The developed method was used for quantification of seven active components in Zhuanggu Guanjie pills,which had advantage of accuracy,repeatability and simplicity.

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