RP-HPLC法同时测定芫花药材中7种黄酮类成分的含量___

目的：建立RP-HPLC法同时测定芫花药材中芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素和芫花素含量。方法：采用Kromasil C₁₈柱(250 mm×4.6 mm,5 μm)；流动相为甲醇-0.05% 磷酸水溶液梯度洗脱，流速1.0 mL · min^-1；检测波长为338 nm，柱温40 ℃。结果：在上述条件下，芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素和芫花素的质量浓度分别在1.21~12.1μg·mL^-1(r=0.9995),0.797~7.97μg·mL^-1(r=0.9997),0.320~3.20μg·mL^-1(^r=0.9993),1.01~10.1μg·mL^-1(r=0.9997),2.02~20.2 μg · mL^-1(r=0.9999),1.13~11.3 μg · mL-1(r=0.9996),1.92~19.2 μg · mL^-1(r=0.9999) 范围内与色谱峰面积呈良好的线性关系，低、中、高浓度的平均加样回收率(n=3)均在96.0 %~100.8 %，RSD均小于2.8 %。结论：该分析方法简便、快速、准确，重现性好，为更好地控制芫花药材的质量提供方法。

 

 

Objective:To establish a method for the determination of genkwainin-5-O-β-D-primeveroside，genkwanin-5-O-β-D-glucoside，luteolin，tiliroside，apigenin，hydroxygenkwanin and genkwanin in Flos Genkwa simultaneously.Methods:The separation was performed on a Kromasil C18column (250 mm×4.6 mm,5 μm) with a gradient elution composed of methanol and 0.05% phosphate acid.The column temperature was set at 40 ℃ with flow rate of 1.0 mL·min-1.The detective wavelength was set at 338 nm.Results:The linear range was 1.21-12.1 μg·mL^-1(r=0.9995),0.797-7.97 μg·mL^-1(r=0.9997),0.320-3.20 μg·mL^-1(r=0.9993),1.01-10.1 μg·mL^-1(r=0.9997),2.02-20.2 μg·mL-1(r=0.9999),1.13-11.3 μg·mL^-1(r=0.9996),1.92-19.2 μg·mL^-1(r=0.9999) respectively (n=6).The average recoveries (n=3) of seven flavonoids were 96.0 %-100.8 % with RSDs of the recoveries (n=9) were less than 2.8 %.Conclusion:The assay demonstrated that the method is simple,rapid,accurate and reproducible for the quality control of Flos Genkwa.

Key word:Flos Genkwa;RP-HPLC;flavonoids;simultaneous quantification

1ChP（中国药典）2005.VolⅠ（一部）：109

2LI Ling-zhi(李玲芝),SONG Shao-jiang(宋少江),GAO Pin-yi(高品一).Research progress in the chemical constituents andpharmacological activities of Daphne genkwa Sieb.et Zucc.(芫花的化学成分及药理作用研究进展).J Shenyang Pharm Univ (沈阳药科大学学报),2007,24(9):587

3ZHANG Bao-xian(张保献)，YUAN Si-tong(原思通)，ZHANG Jing-xiu(张静修),et al.Modern research of Daphne genkwa(芫花的现代研究概况). Chin J Inf Tradit Chin Med(中国中医药信息杂志)，1995,2(10)：21

4LEI Pei-lin（雷沛霖），LI Rao-rao(李娆娆)，YUAN Si-tong(原思通).Chin J Pham Anal(药物分析杂志)，2008，28(5)：834