目的：建立HPLC-ELSD法测定三七血伤宁胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量。方法：采用HYPERSIL C18柱(4.6 mm×250 mm，5 μm)色谱柱，以乙腈(A)-水(B)为流动相进行梯度洗脱\[0~25 min，A-B(20∶80)；25~75 min，A-B(20∶80)→A-B(35∶65)\]，流速1.0 mL·min-1，柱温35 ℃，漂移管温度106 ℃，气体流速2.9 L·min-1。结果：三七皂苷R1在0.225~5.62 μg范围内线性关系良好(ｒ=1.000)，平均加样回收率(n=9)为101.3%；人参皂苷Rg1在2.08~20.8 μg范围内线性关系良好(ｒ=0.9997)，平均加样回收率(n=9)为99.9%；人参皂苷Rb1在2.06~20.6 μg范围内线性关系良好(ｒ=0.9998)，平均加样回收率(n=9)为96.9%。结论：本方法操作简便，结果准确，灵敏度高，重现性好，可作为该产品质量控制的方法。
Key words:HPLC-ELSD;Sanqi Xueshangning capsules;notoginsenoside R1;ginsenoside Rg1;ginsenoside Rb1;assay
Objective:To establish an HPLC-ELSD method for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Sanqi Xueshangning capsules.Methods:The analytical column HYPERSIL C18(4.6 mm×250 mm,5 μm) was adopted;The mobile phase consisted of acetonitrile(A)-water(B) with the gradient elution\[0-25 min,A-B(20∶80);25-75 min,A-B(20∶80)→A-B(35∶65)\],at the flow rate of 1.0 mL·min-1;The column temperature was 35 ℃.The temperature of drift tube was 106 ℃ and the air flow rate was 2.9 L·min-1.Results:The linear range of notoginsenoside R1 was in the range of 0.225-5.62 μg(r=1.000),and the average recovery(n=9) was 101.3%;The linear range of ginsenoside Rg1 was in the range of 2.08-20.8 μg(r=0.9997),and the average recovery(n=9) was 99.9%;The linear range of ginsenoside Rb1 was in the range of 2.06-20.6 μg(r=0.9998),and the average sample recovery(n=9) was 96.9%.Conclusion:This method is simple and accurate.It has high sensitivity and good repeatability.It can be used for quality control of Sanqi Xueshangning capsules.
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8ChP (中国药典)2005.Vol Ⅰ(一部)：10
