Objective To determine of content and entrapment efficiency of Elemene liposomes. Methods The content and entrapment efficiency of Elemene liposomes were determined by Ultra Performance Liquid Chromatography (UPLC). Results Elemene had a good linear relation in a range of 1-100 μg·mL-1. The average entrapment efficiency of Elemene liposomes reached (99.8±0.4)%. Conclusion UPLC method for determination of content and entrapment efficiency of Elemene liposomes was simple, fast and accurate.
1SUN Min-ge(孙敏鸽),LI Shu-bing(李淑斌),ZHOU Li(周莉),et al.Chemical constituents of active pharmaceutical ingredients of elemene(榄香烯原料药的化学成分).J Shenyang Pharm Univ (沈阳药科大学学报),2009,26(8):620
2CHEN Xiao(陈晓),WEI Fu-xiang(魏福祥),QU Yan-hui(瞿延辉).Development in the study of elemene-the active principle of Rhizoma Curcumae(莪术中有效成分—榄香烯的研究进展).Hebei Chem Engin Ind(河北化工),2003,6:9
3YAO Shu-juan(姚淑娟),LIU Bai-yang(刘伯阳),LV Li-yan(吕丽艳).Studies of reducing poison and increasing effect of elemene emulsions on immune function of tumor-bearing mice after chemiotherapy(榄香烯提高肿瘤化疗药物疗效及抗免疫抑制作用的研究).Chin Arch Trad Chin Med (中医药学刊), 2006,24(3):456
4SONG Xiao-dan(宋笑丹),WANG Xue-jun(王学军),DU Xia(杜霞),et al.HPLC determination of the content and entrapment efficiency of β-elemene liposome（HPLC法测定β-榄香烯脂质体药物含量及包封率）.Chin J Pharm.Anal（药物分析杂志）,2007,27(8).1222
5CAO Yue(曹悦),ZUO Dai-ying(左代英),WU Xiao-lan(吴晓兰), et al.Determination of swertiamarin and gentiopicroside in root of Gentiana scabra Bge.by UPLC(超高效液相色谱法测定龙胆药材中的獐芽菜苦苷和龙胆苦苷).Lishizhen Med Mater Med Res(时珍国医国药),2009,20(5):1079
6LIU Mei(刘梅),HAO Gui-xin(侴桂新),ZHANG Mian(张勉), et al.Determination of content of salvianolic acid B and tanshinoneⅡA with UPLC(超高效液相色谱法同时测定丹参药材中丹酚酸B和丹参酮ⅡA的含量).China J Chin Mater Med(中国中药杂志),2008,33(3):319
7LI Qing-wu(李庆武).The experimental study on β-elemene liposomes preparation and its device development(β-榄香烯脂质体的制备工艺研究与生产设备开发).Master degree thesis of Tianjin University(天津大学硕士学位论文),2005.23
