Objective To determine of content and entrapment efficiency of Elemene liposomes. Methods The content and entrapment efficiency of Elemene liposomes were determined by Ultra Performance Liquid Chromatography (UPLC). Results Elemene had a good linear relation in a range of 1-100 μg·mL-1. The average entrapment efficiency of Elemene liposomes reached (99.8±0.4)%. Conclusion UPLC method for determination of content and entrapment efficiency of Elemene liposomes was simple, fast and accurate.
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