毛细管气相色谱法同时测定苍术中茅术醇和β-桉叶醇含量___

摘要 目的: 建立毛细管气相色谱法同时测定苍术中茅术醇与β-桉叶醇含量。方法 HP-INNOWax Polyethylene Glycol(30m×0.32mm×0.5µm)石英毛细管柱，氢火焰离子化检测器(FID)，以正十五醇为内标物，气化室温度270℃，柱温185℃，氮气为载气，流速25mL•min-1。结果 茅术醇在0.102～0.819µg• mL-1范围内呈良好的线性关系(r=0.999 9)，β-桉叶醇在 0.098～0.787µg•mL-1范围内呈良好的线性关系(r=0.999 9)；茅术醇平均回收率为100.9%(RSD=1.80%)，β-桉叶醇平均回收率为99.6%(RSD=1.71%)。结论 本方法简便、准确、重现性好，可用于苍术药材及其饮片的质量控制。

Abstract Objective： To establish gas chromatography method for simultaneous determination of the contents of hinesol andβ-eudesmol in Rhizoma atractylodis. Methods： Chromatography conditions:1-Pentadecanol had been used as the internal standard substance in internal standard method；column: HP-INNOWax Polyethylene Glycol(30m×0.32mm×0.5µm)；detector: Hydrogen flame ionization detector；injection chamber temperature:270℃；column temperature:185℃；carrier gas:N2, the flow rate was 25 mL•min-1.Results： The linear range of hinesol was 0.102－0.819µg•mL-1 (r=0.999 9) and the linear range ofβ-eudesmol was 0.0984－0.787µg•mL-1 (r=0.999 9)；The average recovery rate of hinesol was 100. 9% (RSD =1.80%)，the average recovery rate of β-eudesmol was 99. 6% (RSD=1.71%).Con- clusion This determination method is simple，accurate and reproducible，and suitable for the quality control of the medicinal materials and prepared slices of Rhizoma atractylodis.