HPLC法测定复方甘草麻黄碱片中盐酸麻黄碱和甘草酸的含量___

目的：建立复方甘草麻黄碱片中盐酸麻黄碱和甘草浸膏（以甘草酸计）的质量控制方法。方法：采用C18（ 150 mm×4.6mm，5μm）色谱柱，流动相：0.1%磷酸-乙腈溶液，流速：1.0mL·min-1，柱温：35℃，检测波长：盐酸麻黄碱207nm，甘草酸250nm。结果：盐酸麻黄碱在1.0～101.2 μg·mL-1质量浓度范围内与峰面积呈良好的线性关系Y= 21973.3 X+1421.3（r = 0.9995），回收率为100.6%，RSD= 1.1%（n=3）。甘草酸在14.2～1416.μg·mL-1质量浓度范围内与峰面积呈良好的线性关系Y= 5872.7 X－43188.1（r = 0.9999），回收率99.8%，RSD= 0.8%（n=3）。结论：本方法简便、快速、准确，能有效控制复方甘草麻黄碱片的质量。

Abstract Objective：To establish an HPLC method for the determination of ephedrine hydrochloride and glycyrrhizic acid in compound glycyrrhizin ephedrine tablets． Methods： Chromatogaphic analysis was performed on C18 column(150mm×4.6mm,5μm)with acetonitrile-water(contain 0.1％ phosphorylation)as mobile phase．The flow rate was 1.0mL·min with the detection wavelength at 207 nm (Ephedrine Hydrochloride)、250nm(glycyrrhizic acid)and column temperature at 35 ℃．Results：Ephedrine Hydrochloride,the method showed a good linearity(r=0．9995)in the concentration range of 1.0～101.2 μg·mL-1，whose main recovery rate was 100.6％ ，RSD =1.1％(n=3) ．glycyrrhizic acid,the method also showed a good linearity(r=0．9999) in the concentration range of 14.2～1416.μg·mL-1，whose main recovery rate was 99.8％,RSD =0.8％(n=3) ．Conclusion：The method was simple ,rapid and accurate,so it is proposed for the quality control of compound glycyrrhizin ephedrine tablets．