赤芍药材辐照前后芍药苷含量变化研究___

目的：研究赤芍药材经钴-60射线辐照前后有效成分的含量变化情况。方法：采用HPLC方法，色谱柱为瑞士SPHERIGEL ODS C18(150mm×4.6mm，5μm)柱，流动相为甲醇-水，梯度洗脱(0~10min，15%→20%甲醇；10~30min，20%→30%甲醇)，流速1.0 mL•min-1，检测波长为260.5nm，柱温25℃，分析时间为30min。结果：赤芍药材经钴-60射线辐照后主要有效成分降低近19%。结论：经过系统的方法学考察，该方法具有简便、稳定、可重复性强等特点。可作为赤芍药材辐照前后芍药苷含量变化的评价依据，同时在处理含赤芍药材的中药制剂时应考虑到辐照对主成分的影响。

Abstract Objective:To study the chang of the effective contents in Paeonia lactiflora Pall.befor and after the radiation of Co-60 rays.Methods:The HPLC was adopted.The separation was performed on a Switzerland SPHERIGEL ODS C18(150 mm×46mm，5 μm)column with methanol-water as mobile phase in gradient elution(0-10min,15%→20% methanol;10-30 min,20%→30% methanol).The flow rate was 10 mL·min-1,the detection wavelength was set at 2605nm,and the column temperature was maintaned at 25 ℃.Analysis time was 30 minutes..Results:The effective contents in Paeonia lactiflora had been reduced nearly 20% after the radiation of Co-60 rays.Conclusion:By investigating with systematic methodology,the techcique is convenient,stable and repeatable.It can be regarded as a standard in evaluating the content of paeoniflorin in Paeonia lactiflora before and after the irradiation of Co-60 rays.It also is a reference to the experiments of irradiating Chinese medical herbs.

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