HPLC法测定人血清中利奈唑胺浓度及其临床应用___

目的：建立高效液相色谱法快速测定人血清中利奈唑胺浓度，并用于危重患者治疗药物监测。方法：色谱柱：Hypersil ODS柱；流动相：乙腈-水（23∶77），调整pH至5.0；流速：1.0 mL•min-1；紫外检测波长为254 nm。应用已建立的方法对9例危重患者进行利奈唑胺治疗药物监测，并合理制定个体化给药方案。结果：利奈唑胺在0.31~20.00 mg•L-1浓度范围内线性关系良好（r＝0.9996），最低定量限为0.31 mg•L-1，绝对回收率为47.2%，相对回收率为102.1%，日内、日间精密度的RSD分别<4%和<3.5%。另外，所监测9例危重患者中，有4例患者出现利奈唑胺谷浓度小于最低抑菌浓度的情况。结论：HPLC法简单、快速、准确、灵敏、重现性好，可用于临床利奈唑胺的浓度监测。

Objective:To establish a method of determining serum linezolid in human by HPLC,and use the method for therapeutic drug monitoring in critically ill patients.Methods:Separation was carried out on a Hypersil ODS column,the mobile phase consisted of acetonitrile-water (23∶77),adjust the pH to 5.0 at the flow rate of 1.0 mL•min-1.UV detection wavelength was 254 nm.The method has been used for nine critically ill patients for therapeutic drug monitoring,and individual dosage regimen of linezolid.Results:A good linearity was demonstrated between 0.31-20.00 mg•L-1 (r=0.9996),the lowest limit of quantification of 0.31 mg•L-1.The absolute recovery was 47.2%,and the relative recovery was 102.1%.The intra-day and inter-day deviation was showed by RSD<4%,<3.5%,respectively.In addition,the monitoring of nine critically ill patients,there are four patients with linezolid trough concentrations less than the minimum inhibitory concentrations.Conclusion:The method is simple,rapid,accurate,sensitive,reproducible,and validated for determination of serum linezolid in clinical practice.