HPLC法同时测定三七花中人参皂苷Rb1和三七皂苷Fe的含量___

目的：建立HPLC法同时测定三七花中人参皂苷Rb1和三七皂苷Fe 2种皂苷类成分的含量。方法：采用Promosil-C18(4.6 mm×250 mm,5 μm)色谱柱，流动相为乙腈-0.05%磷酸水溶液，梯度洗脱，流速1.0 mL•min-1，检测波长203 nm，柱温30 ℃。结果：人参皂苷Rb1和三七皂苷Fe分别在51.21~307.3 μg•mL-1(r=0.9995,n=6)和50.94~305.6 μg•mL-1(r=0.9998,n=6)范围内呈良好线性关系；平均回收率(n=3)分别为97.9%~98.9%和97.0~99.4%。结论：本测定方法简便、快速、准确，为三七花质量评价提供了依据。

Objective:To develop an RP-HPLC method for simultaneous determination of ginsenoside Rb1 and notoginsenoside Fe in flower buds of Panax notoginseng.Methods:The analysis was performed on a C18 column (4.6 mm×250 mm,5 μm) with gradient elution of acetonitrile-0.05% phosphoric acid solution at a flow rate 1.0 mL min-1.The UV detection wavelength was 203 nm,and the column temperature was set at 30 ℃.Results:The linear range was 51.21-307.3 μg•mL-1(r=0.9995,n=6) for ginsenoside Rb1 and 50.94-305.6 μg•mL-1(r=0.9998,n=6) for notoginsenoside Fe.The average recoveries(n=9) of ginsenoside Rb1 and notoginsenoside Fe were 97.9%-98.9% and 97.0-99.4% respectively.Conclusion:The method is found to be simple and accurate for simultaneous analysis of ginsenoside Rb1 and notoginsenoside Fe in flower buds of Panax notoginseng,and provided a reliable way for evaluating the quality of it.

1SONG Jian-ping(宋建平),ZENG Jiang(曾江),CUI Xiu-ming(崔秀明), et al.Studies on chemical constituents from rhizomes of Panax notoginseng (Ⅱ)(三七根茎的化学成分研究(Ⅱ)).J Yunnan Univ(云南大学学报),2007,29 (3):287
2XU Cong(胥聪),WEI Jun-xian (魏均娴).A study on the chemical constituents of the essential oils from flower-buds of Panax notoginseng(三七花挥发油的化学成分研究).West China Med J(华西药学杂志),1992,7(2):79
3Gao Xianfu,Dan Mo,Zhao Aihua,et al.Simultaneous determination of saponins in flower buds of Panax notoginseng using high performances liquid chromatography.Biomed Chromatogr,2008,22:244
4ZHOU Ying-chun(周迎春),ZHAO Huan-qing(赵怀清),LIANG Ning(梁宁),et al.Determination of the contents of ginsenoside Rg1,Re,Rb1 and the notoginsenoside R1 in the total notoginsenosides of the Panax notogineseng (Burk.) F.H.Chen by HPLC(高效液相色谱法同时测定三七总皂苷中人参皂苷Rg1、Re、Rb1与三七皂苷R1的含量).J Shenyang Pharm Univ(沈阳药科大学学报),2003,20(1):27
(本文于2009年5月12日修改回)