多波长高效液相色谱法同时测定桂枝汤中5种有效成分的含量___

目的：利用多波长高效液相色谱法，建立同时测定桂枝汤中5种有效成分(芍药苷、甘草苷、肉桂酸、桂皮醛和甘草酸)含量的检测方法。方法：采用Diamond C₁₈色谱柱(4.6 mm×250 mm，5 μm)，柱温25 ℃；0.1%磷酸溶液(A)-乙腈(B)为流动相，以1 mL·min^-1梯度洗脱；检测波长：230 nm(检测芍药苷)、250 nm(检测甘草酸)和280 nm(检测肉桂酸、桂皮醛及甘草苷)。结果：在60 min内桂枝汤中芍药苷、甘草苷、肉桂酸、桂皮醛和甘草酸5种有效成分被完全分离；峰面积与其浓度呈良好的线性；加样回收率(n=5)分别为98.7%(RSD=1.4%)，101.7%(RSD=2.1%)，97.1%(RSD=2.4%)，96.4%(RSD=2.5%)，99.4%(RSD=1.9%)。结论：利用多波长高效液相色谱法，建立了一种可靠的同时测定桂枝汤中5种有效成分含量的检测方法。

Objective: To develop an HPLC method with multiple wavelength ultraviolet detection for the simultaneous determination of peoniflorin,liquiritin,cinnamic acid,cinnamal and glycyrrhizic acid in Guizhi decoction.Methods：The samples were separated on a Diamond C₁₈ column(4.6 mm×250 mm，5 μm),by a gradient elution using 0.1% phosphoric acid(A) and acetonitrile(B) as mobile phase at a flow rate of 1.0 mL·min^-1.The eluate was detected by multiple wavelength:230 nm for peoniflorin;280 nm for liquiritin,cinnamic acid and cinnamal and 250 nm for glycyrrhizic acid.Results：The complete separation was obtained within 60 min for the five compounds(peoniflorin,liquiritin,cinnamic acid,cinnamal and glycyrrhizic acid).Five regression equations showing linear relationships between peak-area and content of each compound were obtained.The average recoveries(n=5) of the compounds listed above were 98.7%(RSD=1.4%),101.7%(RSD=2.1%),97.1%(RSD=2.4%),96.4%(RSD=2.5%),99.4%(RSD=1.9%),respectively.Conclusion：Very satisfactory and reproducible results are obtained for the simultaneous determination of the five compounds in Guizhi decoction.