Objective:To establish an HPLC method for chiral separation of pinocembrin enantiomers;to monitor the process of separation reaction of pinocembrin and to determine the optical purity of the product.Methods:RP-HPLC with Chiralpak AD-RH chiral column was adopted.The mobile phase was 100% methanol at the flow rate of 1.0 mL·min-1,and the detective wavelength was 290 nm.Results:The resolution of pinocembrin enantiomers was 5.0 under the above conditions.The method was used for determining the optical purity of the separated enantiomers of pinocembrin.The enantiomeric excess percentage(ee%) was over 97%.Conclusion:With good resolution and repeatability,the method can be reliably used for chiral separation of pinocembrin enantiomers,and determination of the optical purity of S- and R-pinocembrin sample.
1 Hyun K,Pedro LR,Jaime AC,et al.Effects of compounds found in propolis on streptococcus mutans growth and on glucosyltransferase activity.Antimicrob Agents Chemother,2002,46(5):1302
2 Jung JH,Pummangura S,Chaichantipyuth C,et al.Bioactive constituents of Melodorum fruticosum.Phytochemistry,1990,29 (5):1667
3 Khushbaktova ZA,Yusupova SM,Zamaraeva MV,et al.Interrelationships of the structures and antioxidant activities of some flavonoids from the plants of Central Asia.Chem Nat Compd,1996,32(3):338
4 Zhu XM,Fang LH,Li YJ,et al.Endothelium-dependent and -independent relaxation induced by pinocembrin in rat aortic rings.Vascul Pharmacol,2007,46 (3):160
