萎胃宁浓缩丸定性定量方法研究___
Study on quality and quantity methods of Weiweining pills
分类号：
出版年·卷·期（页码）：2009,29 (12):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的：建立萎胃宁浓缩丸定性定量方法。方法：采用TLC法鉴别方中黄芩、黄连、白芍、木香、甘草；采用HPLC法测定芍药苷含量，色谱柱为Phenomenex Luna C18柱(250 mm×46 mm,5 μm)，流动相为乙腈-002%磷酸(15∶85)，流速10 mL·min-1，检测波长230 nm；柱温25 ℃。结果：定性鉴别薄层色谱特征明显；芍药苷浓度在614~1228 μg·mL-1范围内线性关系良好(r=09997),平均回收率(n=5)为977%，RSD=17%。结论：该法可以准确地对萎胃宁浓缩丸进行定性、定量,有效地控制该制剂的质量。
-----英文摘要：---------------------------------------------------------------------------------------
Objective:To establish the quality and quantity methods of Weiweining concentrated pills.Methods:Radix Scutellariae,Rhizoma Coptidis,Radix Paeoniae Alba,Radix Aucklandiae,Radix et Rhizoma Glycyrrhizae in this preparation were identified by TLC.The content of paeoniflorin in Weiweining concentrated pills was determined by HPLC.HPLC method was performed on a Phenomenex Luna C18 column(250 mm×46 mm,5 μm) with a mobile phase of acetonitrile-002% phosphoric acid(15∶85).The wavelength of detector was set at 230 nm and the column temperature was 25 ℃.Results:Characteristics of identification were very obviously.The linear range of paeoniflorin was 614-1228 μg·mL-1(r=09997) and the average recovery was 977%（RSD=17%）.Conclustion:The methods of qualitative and quantitative analysis are accurate and suitable for effectively controlling quality of Weiweining concentrated pills.
-----参考文献：---------------------------------------------------------------------------------------
1DENG Yi（邓沂），WEI Shu-chang（魏舒畅），JIN Hui（金辉）.HPLC determination of peoniflorin in Yushi Weiweining concentrated pills（高效液相色谱法测定于氏萎胃宁浓缩丸中芍药苷的含量）.Chin Arch Tradit Chin Med(中医药学刊),2004，22(6):1023
2ChP(中国药典).2005.Vol Ⅰ(一部)：211，42，69
3DING Li-xia(丁丽霞),CHANG Xin-quan(常新全).Analysis Handbook about the Active Ingredients of Chinese Medicine(中药活性成分分析手册).Vol Ⅰ(上册).Beijing(北京)：Xueyuan Press(学苑出版社),2002457
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