宽叶羌活超临界CO2流体萃取工艺研究___

目的：优化宽叶羌活挥发油超临界CO2萃取工艺。方法：利用超临界CO2流体萃取技术(SFE-CO2)，在固定的萃取时间2 h和气体流量65 kg·h-1下，研究萃取温度和萃取压力对宽叶羌活挥发油提取率的影响，并采用气相色谱-质谱联用技术 (GC-MS)进行分析萃取物的化学成分，以建立宽叶羌活挥发油提取的最佳工艺条件。结果：宽叶羌活挥发油提取的最佳工艺条件为萃取温度50 ℃、萃取压力25 MPa，萃取时间2 h，气体流量65 kg·h-1，挥发油提取率达880%，约为水蒸气蒸馏法(SD)的6倍。超临界萃取挥发油中共鉴定出159种化合物，其中含Cl、S、B、F、Si的化合物在相关研究中均未见报道。结论：温度和压力是影响超临界CO2萃取宽叶羌活挥发油提取效率和稳定性的关键。

Objective:To optimize the extracting process for Notopterygium forbesii by supercritical CO2 extraction.Methods:The best conditions of the extracting essential oil from root of Notopterygium forbesii by SFE-CO2 were studied and chemical composition was determined by using the GC-MS.Results:The optimum process was as following:The temperature of extracting technique was 50 ℃，pressure 25 MPa，extraction time 2 h and gas flow rate 65 kg·h-1;The extracting rate of essential oil was 88%,about 6 times to steam distillation.And 159 kinds of compound was identified from extracting essential oil,furthermore compound containing Cl,S,B,F,the Si element has not been reported in the related literature.Conclusion:Temperature and pressure are the key factors which affect extracting efficiency and stability of Notopterygium forbesii by supercritical CO2 extraction.