毛细管气相色谱法同时测定酚氨咖敏颗粒中的4个组分含量___

目的：建立毛细管气相色谱法同时测定酚氨咖敏颗粒中对乙酰氨基酚、氨基比林、咖啡因和马来酸氯苯那敏的含量。方法：以无水乙醇为溶剂，样品无需衍生化处理，直接进样，用SE-30大口径毛细管色谱柱(30 m×053 mm,10 μm)，FID检测器，以盐酸麻黄碱为内标，进行测定。结果：对乙酰氨基酚、氨基比林、咖啡因和马来酸氯苯那敏的线性范围分别为20~2000 μg·mL-1、15~1500 μg·mL-1、10~500 μg·mL-1、20~100 μg·mL-1，各组分的平均回收率在989%~1012%范围内，方法的精密度和稳定性良好，RSD< 2%。结论：本法简便、快速、准确，可用于酚氨咖敏颗粒中对乙酰氨基酚、咖啡因、氨基比林和马来酸氯苯那敏的含量测定。

Objective:To establish a gas chromatographic method for the determination of paracetamol,aminopyrine,caffeine,chlorphenamine maleate in paracetamol,aminophenazone phenacetin,caffeine and chlorphenamine maleate granules at the same time.Method:After the sample was dissovled in dehydrated alcohol and got filtered.Analysis the filtrate directly.The GC system included SE-30 wide bore capillary column (30 m×053 mm,10 μm) with flame ionization detector (FID),ephedrine hydrochloride was used as internal standard.Results:The linear range of paracetamol,aminopyrine,caffeine and chlorphenamine maleate was 20-2000 μg·mL-1,15-1500 μg·mL-1,10-500 μg·mL-1and 20-100 μg·mL-1,respectively;The average recoveries were 989%-1012%;The precision and stability of the method were good,RSD < 2%.Conclusion:The method is convenient,rapid,accurate and reliable and may be adopted for assay of paracetamol,aminopyrine,caffeine and chlorphenamine maleate in paracetamol,aminophenazone phenacetin,caffeine and chlorphenamine maleate granules.

 

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