毛细管气相色谱内标法同时测定藏木香药材中土木香内酯、异木香内酯的含量___

目的：测定藏木香药材中土木香内酯、异土木香内酯的含量。方法：用SE-30毛细管柱（30m×0.32mm×0.25μm）；检测器温度为260℃（FID） ；进样口温度240℃程序升温：初始温度50℃，以20℃.min-1的速率升温至170℃，保持28min，再以25℃.min-1的速率升温至250℃，保持1min；以尼泊金丁酯为内标物定量。结果：土木香内酯与异土木香内酯分离良好，在0.6mg•mL-1～4.2 mg•mL-1范围内，呈良好的线性关系（r=0.9999）。结论：本法简便、灵敏、专属性强、重复性好，可用于藏木香药材的含量测定，控制内在质量。关键词：藏木香；气相色谱法；土木香内酯；异土木香内酯

 Objective：A capillary GC method was established for the determination of alantoctone and isoalantoctone in zangmuxiang. Methed：The analytical column was SE-30 capillary column（30 m×0.32 mm×0.25 μm）with an FID detector . The injector temperature was 240 ℃. The detector temperature was set at 260 ℃. The column temperature was programmed from 50 ℃ at a rate of 20 ℃•min-1 to 170 ℃(holding 28 min), then raised to 250 ℃ at 25 ℃•min-1. The final temperature was maintained for 1 min. Results：The linear ranges of alantoctone and isoalantoctone were 0.6 μg-4.2 μg （R2=0.9999），which showed good linearity. Conclution：The methed is simple, specificity, accurate and reliable, and can be used for quality control of Zangmuxiang.