HPLC同时测定三七花中鸟苷和腺苷的含量___

Objective : An RP - HPLC method was developed to determine the contents of guanosine and adenosine simultaneously inflower buds of Panax notoginseng . Methods : The analysis was performed on a C₁₈ column with gradient elution of acetonitrile and water at a flow rate 1.0 mL · min ^-1 .The UV detection wavelength was 254 nm , and the column temperature was set at 30 ℃ . Results : The linear range was 17.2 - 103.2 μg · mL ^-1 ( r = 0.9995 , n = 6 ) for guanosine and 15.6 - 93.6 μg · mL ^-1 ( r = 0.9997 , n = 6 ) for adenosine . The average recoveries of guanosine and adenosine were 97.6 % - 99.7 % ( n = 3 ) and 98.1 - 99.2 % ( n = 3 ) respectively . Conclusion : The method was found to be simple and accurate for simultaneous analysis of guanosine and adenosine in flower buds of Panax notoginseng , and provided a reliable way for evaluating the quality of it .