HPLC-MS/MS法测定人血浆中莫沙必利及其代谢物含量___

Objective: To establish a HPLC-MS/MS method for the determination of mosapride(MS) and its metabolite(M1) in human plasma .  Methods: Phenomenex Gemini C₁₈ column (50mm×3.0 mm，3 µm) was used. The mobile phase was methanol-0.04% formic acid (58 : 42). The alkalinized sample was extracted with acetic ether and the content of drug was determined by HPLC-MS/MS using electrospray ionization.  MS, M1 and Cisapride(IS) were detected on multiple reaction monitoring(MRM) mode by the transitions from the precursor to the production (m/z: 422.1/198.0, 314.2/198.1 and 466.2/184.0).  Results: For MS, calibration curve was linear in range from 0.25 to 128µg·L^-1 (r = 0.9999), the limit of quantitation in plasma was 0.25µg·L^-1, the method recovery was 99.8 %-103.5 %, the RSD of inter- and intra-day precision were less than 5.2 % and 6.4 %, respectively.  Conclution: This method is found to be rapid, sensitive and accurate for determination of MS and its metabolite in human plasma.