不同方法提取的莲子心挥发油气质联用成分分析___

Objective：To compare the chemical constituents of the essential oil from Plumula nelumbinis by supercritical fluid extraction and steam distillation by using the GC-MS Analysis. Methods: The essential oils were extracted from the plumule of Nelumbo nucifera Gaertn. by supercritical fluid extraction (SFE) and steam distillation (SD). The amounts of the components from the essential oil were determined by normalization method. The components were separated and identified by GC-MS. Chromatographic conditions were: Capillary column HP-5MS 5% Phenyl Methyl Siloxane Capillary 30.0m×250μm×0.25μm nominal; Injector temperature 250℃;Program temperature: initial column temperature 50℃,maintained 3 min, and increased to 250℃ at the rate of 4℃·min^-1,held 7 min; Splitless injection. Result: There were 25 components from Plumula nelumbinis by SFE which separated and identified by GC-MS. The major constituents are n-Hexadecanoicacid(21.06%),2-Chloroethyllinoleate(42.85%) and (Z)-,9,17-Octadecadienal (11.99%). There were 36 components from Plumula nelumbinis by SD. The major constituents are Pentadecanoic acid, 14-methyl-, methyl ester (11.43%),8,11-Octadecadienoic acid, methyl ester (8.95%) and Pentadecane（6.89%）. Conclusion: The result got by SFE method can reflect the chemical components of the medicinal materials more accurately and more comprehensively.