铈（Ⅳ）-抗坏血酸-奎宁化学发光体系的研究和应用___

目的：建立一种Ce(Ⅳ)-抗坏血酸-奎宁化学发光体系快速测定奎宁的化学发光分析新方法。方法：在硫酸介质中，抗坏血酸可以显著增敏Ce(SO4)2-奎宁产生的化学发光，从而试验以流动注射化学发光法测定奎宁注射液。结果：在优化的试验条件下，该法测定奎宁的线性范围为1.0×10-6～1.0×10-4g•mL-1，检出限为3.7×10-7g•mL-1，其RSD为1.6%(c=5.0×10-6 g•mL-1,n=11)。结论：方法的选择性较高,线性范围较宽,并成功应用于奎宁针剂含量的测定,扩大了具有荧光特性药物的分析范围。

Objective:a new chemiluminesence method combined with flow injection technique was developed for the determination of quinine.Method:ascorbic acid could greatly enhance the CL intensity of quinine and Ce(IV) in vitriol as a medium.,thus,Quinine injection was determined with t chemiluminesence method combined with flow injection technique.Result:Under the optimized conditions,the linear range for quinine was 1.0×10-6-1.0×10-4 g•mL-1 with a detection limit of 3.7×10-7 g•mL-1and the relative standard deviation was 1.6% (c=5.0×10-6 g•mL-1,n=11).Conclusion:Quinine injection was successfully determined with the suggested method in enlarged analytical range by fluorescence characteristic due to its more higher selectivity and its wider linear range.