高效液相离子排阻色谱法测定血液透析液中醋酸根含量___

目的：建立一种测定血液透析液中醋酸根含量的方法。方法：采用高效液相离子排阻色谱法测定血液透析液中醋酸根的含量。色谱条件：色谱柱为Rezex ROA-Organic Acid H+（150 mm×7.8 mm，8 μm） 流动相为0.0025 mol•L-1硫酸溶液，流速：0.5 mL•min-1 检测波长：220 nm。结果：醋酸根线性范围是1.64～6.54 mmol•L-1（r=0.9994），平均加样回收率为100.6%，RSD=0.08% (n=5)。结论：此方法操作简便，测定时间短，准确度高，透析液中其他离子对测定影响小。

Objective:To introduce a determination method of acetate in dialysate.Method:High liquid chromatography (HPLC) was used to determine acetate.A under the chromatographic conditions:Rezex ROA-Organic Acid H+（150 mm×7.8 mm，8 μm）column mobile phase was 0.0025 mol•L-1 sulfuric acid solution at a flow rate of 0.5 mL•min-1,detection wavelength was 220 nm.Results:The linear correlation of acetate was 1.64-6.54 mmol•L-1 with correlation coefficient of 0.9994.The average recovery was 100.6%,RSD=0.08%(n=5).Conclusions:The results showed that the method is accurate,simple and rapid for analysis of acetate in dialysate.