巴戟天中菊淀粉型五聚糖的分离与制备___
Isolation and preparation of inulin-type pentasaccharide in Morinda officinalis How
分类号：
出版年·卷·期（页码）：2009,29 (10):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的：从巴戟天寡糖粗提取物中分离、制备菊淀粉型五聚糖。方法：使用Phenomenex synergi 4 μ Hydro-Rp 80A 色谱柱（250 mm×30 mm，4 μm），以甲醇-水（2∶98）为流动相，蒸发光散射检测器检测，分离、制备菊淀粉型五聚糖，并根据波谱数据对其进行结构鉴定。结果：从巴戟天寡糖粗提取物中分离得到菊淀粉型五聚糖纯品，得率1.8%，纯度99.99%。结论：本实验条件方法简便，重现性好。
-----英文摘要：---------------------------------------------------------------------------------------
Objective:To isolate and preparate the inulin-type pentasaccharide from oligosaccharide extract of Morinda officinalis How.Method:Preparative liquid chromatography with a Phenomenex synergi 4 μ Hydro-Rp 80A(250 mm×30 mm，4 μm) column as the stationary phase,methanol-water(2∶98) as the mobile phase and detector(ELSD) as the detector was used for isolation and preperation of inulin-type pentasaccharide and the structrue of this compound was elucidated by spectroscopic data.Result:The compound inulin-type pentasaccharide was preperated with the high purity of 99.99%,and the yield rate was about 1.8%.Conclusion:The proposed method is simple and repeatable.
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