UPLC法测定同仁乌鸡白凤丸中芍药苷的含量___
UPLC determination of paeoniflorin in Tongren Wuji Baifeng pills
分类号：
出版年·卷·期（页码）：2009,29 (9):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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-----英文摘要：---------------------------------------------------------------------------------------
Objective:To develop an ultra-performance liquid chromatography(UPLC) method for the determination of paeoniflorin in Tongren Wuji Baifeng pills.Methods: A Waters ACQUITY UPLCTM BEH C18 column(2.1 mm×50 mm,1.7 μm) was adopted with the mobile phase of methanol-water(20∶80) at a flow rate of 0.4 mL·min-1.The detecting wavelength was set at 232 nm, and the column temperature was 35 ℃. Results: The calibration curve of paeoniflorin was linear in the range of 9.92-99.2 ng(r=0.9999), the mean recovery(n=6) was 102.0% with RSD 2.0%, the time for a analysis program was about 5 minutes. Conclusion: Comparing with the HPLC method, the UPLC method for determination of paeoniflorin in Tongren Wuji Baifeng pills increases the analysis speed and efficiency;it can also increase the analysis sensitivity and save the solvents.
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1 Drug Specifications Promulgated by the Ministry of Public Health, P R China(卫生部药品标准). WS3-B-3185-98
2 NIE Li-xing(聂黎行),WANG Gang-li(王钢力),LI Xiang-ri(李向日),et al.HPLC determination of paeonin and denshensu in Tongren Wuji Baifeng pills(HPLC法测定同仁乌鸡白凤丸中芍药苷和丹参素的含量). Chin J Pharm Anal(药物分析杂志),2008,28(3)：354
3 Wirth MJ.Mass transport in sub-2-μm high-performance liquid chromatography.J Chromatogr A, 2007,1148(1):128
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