目的：建立赤芝HPLC特征图谱分析方法。方法：采用Alltech C18(150 mm×4.6 mm，5 μm)色谱柱，以乙腈-0.04%甲酸为流动相梯度洗脱(0~10 min时，乙腈0→20%；10~20 min时，乙腈20%→25%；20~50 min时，乙腈25%→30%；50~65 min时，乙腈30%→38%)，流速1.0 mL·min-1，检测波长254 nm，柱温15 ℃。结果：分析了13个批次的赤芝子实体，并建立了三萜酸特征图谱，确定了19个共有峰，其中包括灵芝酸Ⅰ、灵芝酸C2、赤芝酸LM1、灵芝酸G、灵芝烯酸B、灵芝酸B、灵芝烯酸A、灵芝酸A、12-乙酰基-3-羟基-7，11，15，23-四羰基-羊毛甾-8-烯-26-酸、灵芝酸D、赤芝酸A、灵芝烯酸D、灵芝酸C、灵芝酸E、灵芝孢子酸A共15个已知化合物峰以及4个未知化合物峰，有13批赤芝样品特征图谱与对照特征图谱相似度均在0.9以上。结论：本方法简单、准确，重复性好，为赤芝的质量控制标准提供了有效的方法。
Objective:To establish an HPLC method of specific chromatogram analysis on Ganoderma lucidum.Methods:Chromatography conditions were Alltech Alltima C18(150 mm×4.6 mm，5 μm) column，acetonitrile-0.04% formic acid system as mobile phase，gradient elution(0-10 min,acetonitrile 0→20%;10-20 min,acetonitrile 20%→25%;20-50 min,acetonitrile 25%→30%；50-65 min，acetonitrile 30%→38%);detective wavelength at 254 nm，column temperature was 15 ℃.Results:Nineteen common peaks were found in the HPLC specific chromatogram of Ganoderma lucidum，including fifteen known peaks：ganoderic acid Ⅰ,ganoderic acid C2，lucideric acid LM1,ganoderic acid G,ganoderenic acid B,ganoderic acid B,ganoderenic acid A,ganoderic acid A,12-acetoxy-3-hydroxy-7,11,15,23-tetraoxolanost-8-en-26-oic acid,ganoderic acid D,lucideric acid A,ganoderenic acid D,ganoderic acid C,ganoderic acid E,ganosporeric acid A and four unknown peaks.Good similarities with correlation coefficients higher than 0.9 were found in specific chromatograms between the herbs from different sources and standard specific chromatogram，which can be utilized for the identification of Ganoderma lucidum.Conclusion:The method is simple,accurate,and can be used as a quality control method for Ganoderma lucidum.
