液相色谱-串联质谱法同时测定畜禽肉中8种磺胺类药物残留量___

Objective:To establish an LC-MS/MS method to simultaneously determine 8 sulfonamides (sulfadimethoxin,sulfadimidin,sulfamerazin,sulfameter,sulfamethoxazole,sulfapyridine,sulfasomidin, sulfisoxazole) residues in meat. Methods: By adding eprosartan as internal standard, homogenized meat sample was deproteinated with acetonitrile. The analysis was performed in reverse phase liquid chromatography and detected in multiple reaction monitoring mode.Results:In this method, the determination was not effected by endogenous compounds; RSDs of intra- and inter-assay were lower than 10%，LLOQ was 10 ng·g^-1(S/N≥10); Relative recovery of sulfonamide was 86.0%-108.9%; Calibrations between 10-300 ng·g^-1 exhibited consistent linearity and reproducibility.Conclusion:With a simple sample pretreatment, specific and cost-effective assay, the proposed LC-MS/MS method is suitable for sulfonamide residues analysis in meat.