离子色谱抑制电导测定尿液中草酸根、磷酸根及枸橼酸根离子___

Objective:To develop a simple method for the determination of oxalate,phosphate,citrate in human urine by ion chromatography with gradient elution and conductivity detection.Methods:Fresh collective urine samples were centrifuged for 15 min at 5000 r·min^-1 and then the above solution was filtered through a 0.2 μm syringe membrane filter,On-Guard H column and C₁₈ cleanup column before injection.The chromatographic separation was performed on Dionex IonPac AG23(50 mm×4 mm) guard column,and an Dionex IonPac AS23(250 mm×4 mm) analytical column using potassium hydroxide as eluent with a self-regeneration suppressor.Results:This novel method exhibited excellent regression(r≥09998)in the range of 0.5-50 μg·mL^-1,satisfactory precision(RSD less than 3.6% within one day and less than 6.9%day to day).The recovery obtained in the accuracy test was in the range of 96.0%-100.2%.Conclusion:The method is simple,accurate,stable and precise.It can be easily and conveniently adopted for the routine quality control analysis.