HPLC法测定不同规格酸橙枳实中新橙皮苷和柚皮苷的含量___

Objective:To develop an RP-HPLC method for the determination of neohesperidin and naringin in Fructus Aurantii Immaturus of different specifications.Methods:The column was Merck-Lichrospher RP-C₁₈(4.6 mm×250 mm,5 μm);The mobile phase was a mixture of acetonitrile-0.3% phosphoric acid(20∶80),at a flow rate of 1.0 mL·min^-1；The detection wavelength was 283 nm，and the column temperature was 35 ℃.Results:The calibration curves of neohesperidin，naringin were in good linearity over the ranges of 0.774-3.87 μg(r=0.9997),0.816-4.08 μg(r=0.9997),and the average recoveries(n=6) of neohesperidin and naringin were 99.4%,99.9% with RSD of 1.3%,1.6% respectively.Neohesperidin and naringin contents in Fructus Aurantii Immaturus of different specifications were 14.6%,7.02% (diameter 0.5-1.0 cm); 12.3%,9.78%(diameter 1.0-1.5 cm); 10.2%,10.2%(diameter 1.5-2.5 cm) respectively. Conclusion:This method is simple，sensitive，accurate，which will supply evidence for the quality control and research of Fructus Aurantii Immaturus.