宁心宝胶囊定性定量方法研究___

Objective：To establish the quality and quantity methods of Ningxinbao capsules. Methods: Ergosterins and nucleosides in Ningxinbao capsules were identified by TLC. The contents of three nucleosides(uridine,guanosine,adenosine) in Ningxinbao capsules were determined simultaneously by HPLC.The chromatographic column was Lichrospher C₁₈ column(250 mm×4.6 mm,5 μm);The mobile phase was methanol-0.05 mol·L^-1 potassium dihydrogen phosphate solution(8∶92) at the flow rate of 1.0 mL·min^-1,and the detecting wavelength was 260 nm.Results:The method of identifying ergosterins and nucleosides was specific. The calibration curves were linear in the ranges of 013-1.90 μg(r=0.9999,n=5) for uridine,0.09-1.30 μg(r=0.9999,n=5) for guanosine, and 0.08-1.21 μg(r=0.9999,n=5) for adenosine;The average recoveries(n=9) were 100.8%,99.4% and 100.9% respectively.Conclusion:The method is specific,effective,sensitive and accurate. It can be used for quality control of Ningxinbao capsules.