麻花秦艽中落干酸和龙胆苦苷的含量测定及麻花秦艽药材HPLC特征图谱的研究___

Objective:To establish an HPLC method for simultaneously determination of loganic acid and gentiopicroside and the HPLC specific chromatogram of Gentiana straminea Maxim..Methods：Chromatographic analysis was achieved on a Diamosil C₁₈ column(250 mm×4.6 mm,5 μm); The mobile phase was acetonitrile-0.1% acetic acid(9∶91) at the flow rate of 1.0 mL·min^-1;The detection wavelength was 250 nm,and the column temperature was 35 ℃.Results：The calibration curves were linear in the range of 72.4-724 μg·mL^-1 for loganic acid(r=0.9996) and 173.4-1734 μg·mL^-1 for gentiopicroside(r=0.9997);The average recoveries of loganic acid and gentiopicroside were 103.7% and 104.9%,respectively.The method provided specific chromatogram with excellent separation and 6 common peaks were comfirmed in specific chromatogram.Conclusion：The method is simple,accurate,creditable and can be applied to the quality control of Gentiana straminea Maxim..