HPLC法测定匹诺塞林含量及有关物质___
HPLC determination of Pinocembrin and related substances
分类号：
出版年·卷·期（页码）：2009,29 (8):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的：采用高效液相色谱法测定匹诺塞林的含量及其有关物质。方法：采用Kromasil C18色谱柱（4.6mm×150mm, 5μm）；以甲醇-水（用磷酸调pH至3.0）（63：37）为流动相；流速1.0mL•min-1；检测波长：290nm；柱温：室温；进样量：20μL。结果：高效液相色谱法测定的线性范围为2~200μg•mL-1；相关系数r =1.0000；日内精密度：RSD为0.10%（n =5）；日间精密度：RSD为0.14%（n = 5）。结论：本法简便快速、准确、专属性好。
-----英文摘要：---------------------------------------------------------------------------------------
0bjective: A HPLC method was established for the determination of pinocembrin and its related substances. Method: The separation was performed on a Kromasil C18 column(4.6mm× 150mm ，5µm).The mobile phase was composed of methanol-water(adjusted to pH 3.0 with phosphoric acid) =63:37 .The flow rate was 1.0mL•min-1 .The detection was carried out with a UV detector at 290nm.The chromatography was conducted at room temperature. The injection was 20µL. Results: The calibration curves were linear between 2~200µg•mL-1 (r = 1.0000).Intra-day and inter-day RSD were 0.10% (n = 5)and 0.14%(n = 5). Conclusion: The method is simple, rapid, accurate and is suitable for the determination of pinocembrin and its related substances.
-----参考文献：---------------------------------------------------------------------------------------
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